Photoautotrophic culture of Chrysanthemum was established under CO2 enrichment (2% v/v). The shoot length and number of leaves were almost equal (2.7 cm and 14 respectively) both under photoautotrophic and photomixotrophic cultures, recorded after four weeks of incubation. Similarly, average dry mass of the plantlets were comparable (31 and 28 mg respectively) under both conditions. The number of branches and internode length which influence the number of propagule potential for mass propagation, were also identical. Nevertheless, photoautotrophic cultivation minimized the risk of contamination in cultures, which in turn will reduce the production cost.