Sorbitol-mediated stabilization of human IgG against thermal inactivation

    loading  Checking for direct PDF access through Ovid

Abstract

Sorbitol at 30% (w/w) stabilized human IgG to thermal denaturation from 60 to 85°C, increasing the protein's half life from 25 to 266 min at 70°C. A kinetic model based on the Lumry-Eyring inactivation scheme was developed and used to estimate the apparent rate constant and activation energy.

Related Topics

    loading  Loading Related Articles