pYH31, a ColE1 compatible His-tagged fusion expression vector

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Abstract

A ColE1-compatible vector was constructed to expressed His-tagged fusion protein in Escherichia coli cells. The vector designated pYH31 is derived from pREP4 and pQE31. It was designed to express simultaneously in the same cell, a His-tagged fused protein and a non His-tagged protein from separate plasmids. The vector was used to express both subunits of the biphenyl dioxygenase oxygenase component together in the same E. coli clone.

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