The aim of this study was to assess the influence on the infrared laser fluorescence response of some storage methods commonly used in dental research. Forty extracted permanent teeth, selected from a pool of frozen teeth, were divided into four groups of 10. Three groups were stored at 4°C in 1% chloramine, 10% formalin or 0.02% thymol solution. The fourth group was stored at −20°C (no storage solution added). Fluorescence measurements were performed at 14, 77, 113, 168, 232, 486 and 737 days. After 2 years, significant decreases in fluorescence (p < 0.01) for the samples in formalin (–60%), chloramine (–72%) and thymol (–54%) were observed. The frozen teeth showed a slight but non-significant increase in fluorescence of 5% (p > 0.01). Storing solutions have a significant influence on the fluorescence yield. Samples used for in vitro purposes stored frozen do not significantly change their fluorescence response. Thus, cut-off values obtained under the latter conditions could be extrapolated to the in vivo situation.