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Mutations in the myosin XVA gene (MYO15A) cause congenital non-syndromic deafness in humans and mice. Therefore, the MYO15A gene represents a candidate gene for hereditary hearing loss in dogs. Using a human cDNA to screen a dog BAC library, we isolated a canine BAC clone. Sequencing of the BAC ends confirmed homology to the human gene. To facilitate future linkage studies, we report the physical mapping of the canine MYO15A gene to CFA5q23-q24 by FISH and RH mapping.