Increased Vascular Responsiveness to Norepinephrine in Rats With Heart Failure Is Endothelium Dependent: Dissociation of Basal and Stimulated Nitric Oxide Release

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Abstract

Background

Endothelial dysfunction and abnormal vascular responsiveness to vasoconstrictors may play an important role in chronic heart failure (CHF). The purpose of our study was to (1) evaluate whether the vascular response to norepinephrine is abnormal in a rat model of heart failure; (2) investigate the role of α1- and α2-adrenergic receptors; and (3) assess the contribution of the endothelium, and specifically endothelium-derived nitric oxide, to this response.

Methods and Results

Concentration-response curves of rat thoracic aortic rings were studied in isolated organ baths at 1 week after coronary artery ligation. In CHF rats, norepinephrine- induced contractions were increased in intact rings compared with rings from sham rats, despite decreased contraction in denuded rings. Decreased α1-receptor sensitivity was demonstrated by the increased EC50 of methoxamine in endotheliumdenuded rings from CHF rats, although maximal responses to KCl contraction were also decreased in CHF. There was no difference in the vascular response to clonidine, and acetylcholine- mediated relaxations were preserved in CHF rats, suggesting normal stimulated nitric oxide release. However, nitric oxide synthase inhibition with Nω-nitro-L-arginine methyl ester, as well as measurements of basal cGMP, demonstrated that basal nitric oxide release was decreased in CHF rats.

Conclusions

This study demonstrates that the increased vascular responsiveness to norepinephrine in intact vessels from rats with heart failure is the result of decreased basal nitric oxide release and suggests that the dissociation of basal and stimulated nitric oxide release may play a pathophysiological role at an early stage of heart failure.

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