Abstract 17958: A Novel Method of Reducing Hypoxic Injury by Perfluorocarbon Nanobubbles in Cardiomyocytes During Hypoxia Treatment - miRNA Reflections

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Abstract

Introduction: To identify the role of perfluorocarbon nanobubbles in cardioprotection after hypoxic injury.

Hypothesis: PFC nano-bubbles could have a potential role in myocardial salvage during acute ischaemia.

Methods: Perfluorocarbon nanobubbles were synthesised by a standard protocol. Cardiomyocytes were seeded in cell culture wells and were subjected to hypoxia treatment (5% O2). The cells were treated with PFC nanobubbles or Phosphate buffered saline which served as a control. PFC's were added to the hypoxia treatment cells as well as to control cells without hypoxia treatment. Serial additions of PFC’s were made for 4 hours during hypoxia treatment. At the end of the procedure the cells were analysed by microscopy and mi RNA was extracted by a standard protocol. The complete panel of mi RNA was quantitatively and qualitatively assessed. The concentration of total RNA measured by nano drop was >475 ng/microL (RNA Integrity Number >9.8, for each).

Results: Compared to the controls there was a significant increase and decrease of certain miRNA’s. Some apoptosis signaling RNA’s and miRNA’s signaling cardiac failure were reduced. There was increase in certain cardioprotective miRNA’s also. The table below shows some miRNA's of the hypoxia vs. normoxia in PFC treated cells.

Conclusions: There is potential for the role of PFC nanobubbles in the treatment of acute myocardial ischemia. Further invivo and invitro studies are required to study the pathways and therapeutic benefits.

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