Effect of RNAi-mediated gene silencing of Livin on apoptosis of human breast cancer cell line ZR-75-30

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Abstract

Objective

To explore the effects of Livin gene knock down using sequence-specific siRNA on apoptosis of human breast cancer cell ZR-75-30.

Methods

Chemically synthesized double stranded RNA(dsRNA) targeting Livin was transfected into human breast cancer cell ZR-75-30 with lipofectamine™2000. The transfection efficiency was observed under a fluorescence confocal microscope. Expression of Livin at both mRNA and protein levels were detected by reverse transcription-polymerase chain reaction(RT-PCR) and immunohistochemical analysis. The effects on apoptosis of ZR-75-30 cells were assessed by FCAS.

Results

The Livin siRNA can effectively and specifically inhibited the expression of Livin gene in ZR-75-30. The inhibition rate was 53.66% at mRNA level and 58.32% at protein level. After 24h, (8.36±0.20)%cells transfected with siRNA were induced to apoptosis.

Conclusion

Chemically synthesized short Livin-siRNA can effectively inhibit Livin over expression and remarkably induce apoptosis in human breast cancer cell line ZR-75-30. Livin RNAi has a potential value in gene therapy of breast cancer.

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