Vascular endothelial growth factor promotes peripheral nerve regeneration after sciatic nerve transection in rat

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Abstract

Objective:

To evaluate the local effect of vascular endothelial growth factor (VEGF) on transected sciatic nerve regeneration.

Methods:

Sixty male white Wistar rats were divided into four experimental groups randomly (n=15). In transected group the left sciatic nerve was transected and the stump was fixed to adjacent muscle. In treatment group the defect was bridged using a silicone graft filled with 10 μL VEGF. In silicone group the graft was filled with phosphate-buffered saline. In sham-operated group the sciatic nerve was exposed and manipulated. Each group was subdivided into three subgroups with five animals in each and nerve fibers were studied 4, 8 and 12 weeks after operation.

Results:

Behavioral test, functional study of sciatic nerve, gastrocnemius muscle mass and morphometric indices confirmed a faster recovery of regenerated axons in VEGF group than in silicone group (P<0.05). In immunohistochemical assessment, reactions to S-100 in VEGF group were more positive than that in silicone group.

Conclusion:

Local administration of VEGF will improve functional recovery and morphometric indices of sciatic nerve.

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