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A suggestion for a standard procedure to establish biological reference intervals for biochemical quantities by a multicenter approach is presented. This procedure was developed for and used in the Nordic Reference Interval Project 2000 (NORIP). This project established biological reference intervals for 25 frequently requested biochemical quantities through cooperation of 102 Nordic laboratories. Each laboratory performed collection of reference samples and measurement using their routine methods. The bias of each routine method was eliminated by use of common reference materials measured in each of the participating laboratories.