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This article is intended as a guide for the production of biological reference values of healthy people (physiological reference values) by several clinical laboratories using the same measurement system. This guide is a proposal from the Catalan Association of Clinical Laboratory Sciences to be applied worldwide at a regional level. This guide makes it possible for all clinical laboratories in a region using the same measurement system to adopt the same physiological reference limits. The model presented here is based on the assumption that the production of physiological reference values is a professional task that should be shared by both clinical laboratories and the in vitro diagnostics industry.