Capability of new bone formation with a mixture of hydroxyapatite and beta-tricalcium phosphate granules

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The aim of this experimental study was to test a mixture of hydroxyapatite (HA) and beta-tricalcium phosphate (beta-TCP) granules inserted in cranial defects in rabbits, by the evaluation of the hard tissues volume, new bone formation, and residual graft after 4 and 8 weeks.

Material and methods:

Two defects of 8 mm diameter were created at the calvarial bone of 24 Japanese white rabbits for a total of 48 defects. Four groups were created: defects filled with a mixture of HA and beta-TCP granules (test A), defects filled with HA alone (test B), defects filled with beta-TCP (test C), and empty defects (control). Hard tissues volume (remaining graft + new bone) was evaluated by μ-CT and new bone (NB) and remaining graft (RG) percentages were evaluated by histomorphometry. The animals were sacrificed at 4 or 8 weeks postoperatively.


The test groups A, B, and C showed a significant higher total volume compared with controls at 4 and 8 weeks (P < 0.05). Regarding the percentages of NB and RG at 4 and 8 weeks, no significant differences were detected (P > 0.05). When comparing 4 and 8 weeks, test group A showed a significant increase in new bone formation. At both 4 and 8 weeks, no group showed significant differences in NB (P > 0.05). At 8 weeks, test group B had more RG than test group A.


The novel mixture could maintain the volume of the grafted area compared with that with intervention, and in a similar way compared with HA.

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