Sorption of heavy metal (copper and strontium) ions by the meadow chernozem and grey forest soils differing in the content of organic substance was described using the Langmuir equation. The analysis of characteristic sorption coefficients demonstrated that copper possesses the enhanced affinity for the studied soil samples compared to strontium. Maximal values of the sorption of copper (0.085 mmol/g) and strontium (0.045 mmol/g) obtained for the colloidal fraction of meadow chernozem soil (particle diameter d < 1 μm) are approximately 1.5 times larger than for the same fraction of grey forest soil; this ratio remains almost the same even when using the coarser fraction (d < 50 μm). It was established that up to 90% of metal ions could be present in the soil in an immobile form. An increase in the concentration of heavy metal ions in the soil causes their redistribution between the components of absorbing soil complex and an increase in the fraction of metal in mobile forms (water-soluble, exchange, soluble in weakly acidic medium). Upon the microbiological treatment (bioleaching in the suspension variant) of the soils containing copper or strontium ions, the total content of metal, including its mobile forms, decreases by an order of magnitude.