Metabolicprofiles of colonic tissue using ‘Magic angle spinning’ NMR: P037

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Aim:The diagnosis of colonic pathology following endoscopic biopsies relies on histological examination. Magic angle spinning (MAS)proton nuclear magnetic resonance (1H NMR) analyses solid specimens such as tissue, thereby offering a quantitative method of analyzing colonic tissue and aiding diagnoses. The aim of the study was to investigate the metabolic alterations of colonic polyps compared to normal colonic tissue by MAS 1H NMR.Method:Seventeen colonic biopsy specimens were obtained from six patients. Three patients had normal colonoscopies and three had colonic polyps. All 1H MAS NMR spectra were acquired using a Bruker DRX600C spectrometer (Bruker Biospin, Rheinstetten, Germany), operating at a temperature of 283 K. The obtained NMR spectra were analyzed using the software TopsinTM and MATLABTM.Results:Metabonomicprofiling of normal and polypoid colonic tissue from patients revealed a number of metabolites such as lactate, choline and alanine. Changes were seen in choline metabolites.Conclusion:This pilot investigation suggests that MAS 1H NMR could be used for the quantitative metabolic analysis of biopsied tissue for diagnostic and potentiallyprognostic purposes.

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