The osteogenic response to subperiosteal injection of negatively charged ion exchange resins was compared in the tibiae of one-month and 16-to 22-month-old rats. The resins were administered either in the form of beads (CM Sephadex) or as particles (CM cellulose, carboxymethylcellulose), and the animals were killed at two weeks and at one month after injection. Histologically, the resins did not produce an inflammatory response. Periosteal bone formation was observed wherever resin was in contact with bone, and in the resin bed the connective tissues that invested the charged materials ossified within the first month. Marrow spaces commonly formed where periosteal growth was most rapid. The osteogenic effect was independent of resin conformation, and it was more pronounced in the younger rats.