Expression of leptin receptor mRNA (long form splice variant) in the human cerebellum

    loading  Checking for direct PDF access through Ovid


PRIMERS specific to the long isoform of leptin receptor (OB-Rb) mRNA were used in reverse transcriptaselinked polymerase chain reactions (RT-PCR) to investigate the expression of this receptor in the hypothalamus and cerebellum of human and rat. For both regions, we observed RT-PCR cDNAs as well as restriction enzyme cleavage fragments of expected sizes. Additionally, in situ hybridization of human cerebellum using two independent [35S]oligonucleotide probes complementary to the OB-Rb mRNA sequence revealed a prominent hybridization signal within the granular layer. Overall, our findings demonstrate the expression of OB-Rb mRNA in the cerebellum and suggest that in such a location, leptin receptors may mediate a function presumably not linked to body weight homeostasis.

    loading  Loading Related Articles