Effects of ionotropic glutamate receptor blockade and 5-HT1A receptor activation on spreading depression in rat neocortical slices

    loading  Checking for direct PDF access through Ovid

Abstract

THE effect of the AMPA antagonist NBQX (10 μM), NMDA antagonist ketamine (100 μM) and 5-HT1A agonist 8-OH-DPAT (1, 10 and 100 μM) on the properties of a KCl-induced spreading depression (SD) was studied in parietal cortical slices of adult rats. Whereas NBQX did not significantly affect the SD, ketamine significantly (p < 0.01) reduced the amplitude of the first SD peak (12.8 ± 4.6 mV) and blocked the second SD peak when compared with the controls (19.8 ± 5.2 mV and 25 ±5 mV, respectively). Ketamine also decreased the SD duration at half maximal amplitude from 34.9 ± 12.4 s to 22.2 ± 12 s (p < 0.05). 8-OH-DPAT attenuated the duration of the SD from 42 ± 15.6 s to 21.2 ± 10.6 s (p < 0.05, 100 μM). These data indicate that not only NMDA receptor blockade, but also activation of the 5-HT1A receptor attenuates the SD and may be beneficial in the reduction of ischemic injury following focal cerebral ischemia.

Related Topics

    loading  Loading Related Articles