NeuroReport. 18(15):1547-1551, OCT 2007
DOI: 10.1097/WNR.0b013e3282f03f51
,
PMID: 17885599
Issn Print: 0959-4965
Publication Date: 2007/10/01
Intracellular dynamics of calcyon, a neuron-specific vesicular protein
Markus Kruusmägi;Sergey Zelenin;Hjalmar Brismar;Lena Scott;
+ Author Information
aDepartment of Woman and Child Health, Karolinska InstitutetbDepartment of Cell Physics, Royal Institute of Technology, Stockholm, Sweden
Abstract
Calcyon is a brain-specific protein, implicated in clathrin-mediated endocytosis. In this descriptive study we show that calcyon is exclusively expressed in neurons, and localized in moving vesicles. The movement of calcyon-containing vesicles was dependent on temperature and on intact microtubules, in addition these vesicles were colocalized with a marker for endocytosed plasma membrane proteins, suggesting that calcyon vesicles follow the endocytic recycling pathway. We also show using evanescent wave microscopy that there is a pool of ready releasable calcyon vesiclesaccumulated beneath the plasma membrane. We conclude that the mobility and storage properties of calcyon-containing vesicles imply that they play a role in brain plasticity.
