Issn Print: 1058-2916
Publication Date: 2004/03/01
A NEW METHOD FOR REAL-TIME DETECTION OF THE OCCURRENCE AND LOCATION OF HEMOLYSIS
S Mochizuki; K Takiura; Y P Sun; T Chinzei; Y Abe; K Imachi
+ Author Information
Author Information: Department of Biomedical Engineering, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
Excerpt
Present methods that evaluate the hemolysis in blood pumps employ circulation of blood and measurement of free hemoglobin. It cannot evaluate the occurrence and location of hemolysis. In this study, a new method was developed to detect where hemolysis occurs in real time. The chemical IL/minescence of LL/mmol and 02 were catalyzed by the Fe complex as hemoglobin. Hemoglobin from the hemolytic erythrocyte renders this reaction, which generates photon emission. The hemolysis can be detected by photon emission counts. A small amount of Luminol and H2O2 were added to goat blood. Water was added to this mixture to generate hemolysis while the photon was quantified every 100msec with a photo multiplier tube. Hemoglobin solutions (0μg/dI to 100μg/dl in goat plasma) were added to a mixture of Luminol and H2O2, and the photons were counted at each concentration of hemoglobin. A centrifugal blood pump (HPM-05, Nikkiso) filled with goat blood, Luminol and H, O, while the photons were counted when pump drive. In the goat blood with Luminol and H2O2, the photon counts were about 100counts/100msec, as same as that counted in dark box. After water added into the mixture, the photon emission immediately increased, and the maximum peak of 1673counts/100msec at 9.5sec. It returned to initial value at 20sec. The hemoglobin concentration was estimated from the photon count values. Photon emission at pump head increased while driving the pump. An optical fiber scanning the pump is presently used to measure the emission of photons at micro-points to determine where hemolysis occurs in real time.
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