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Lateral-wedged insoles have been shown to help clinically alleviate pain associated with medial compartment osteoarthritis. This study analyzed the effects of lateral-wedged insoles on the gait and medial knee compartment load of 17 healthy subjects. Three-dimensional gait analysis was performed for each subject with and without wearing a 5° lateral-wedged insole. Subjects walked at a constant velocity for both conditions. A motion analysis system and force plate were used to calculate temporal and spatial parameters, joint angles, moments, and powers. An analytical model was developed to estimate medial compartment loads at the knee for each subject during both conditions. Results were compared with a Student's paired t test. There were no significant differences in temporal and spatial parameters, joint angles at the hip, knee, and ankle, or kinetics at the hip and ankle. However, the external varus moment and estimated medial compartment load at the knee were reduced significantly with the addition of the lateral-wedged insole. These results suggest that the pain relief and improvement in function reported by patients with osteoarthritis while using lateral-wedged insoles may be achieved by a reduction in external varus moment and medial compartment load.