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Excerpt
Introduction: Hepatic injury following I/R is attributed to the development of oxygen free radical (OFR) mediated lipid peroxidation. The use of free radical scavengers can offer significant protection against OFR induced liver injury. We hypothesized that a new potent OFR scavenger, polyethylene glycol-superoxide dismutase (PEG-SOD), can inhibit OFR mediated lipid peroxidation in hepatic I/R injury. Methods: Twelve male Sprague-Dawley rats (300-350g) were subjected to occlusion of the left and middle hepatic vessels for 90 minutes followed by 120 minutes of reperfusion. PEG-SOD (5000 units/kg) was given IV to six rats prior to vascular occlusion and again immediately upon reperfusion. Normal saline (NS) was given to the remaining six rats to be used as external controls. The right hepatic lobe and left hepatic lobe were harvested separately and tissue malondialdehyde (MDA), an end product of lipid peroxidation, was measured by spectrophotometry. Results: A significant increase in MDA was found in the control group after two hours of reperfusion. Treatment with PEG-SOD inhibited the rise in tissue MDA (see chart below). (Figure 1)
Conclusion: PEG-SOD can effectively attenuate hepatic ischemia/reperfusion injury by inhibiting OFR mediated lipid peroxidation.
