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The mechanisms involved during sepsis-induced immunosuppression are far from being extensively established. The objective of the present study was to investigate whether two characteristics of T cells were altered in this situation: the percentage of circulating γδ T lymphocytes and the level of CD3 expression on T lymphocytes.Observational study.Adult intensive care units in a university hospital.Patients with septic shock (n = 21) and healthy individuals (n = 21).None.In patients, we first observed the decreased percentage of γδ T lymphocytes in peripheral blood (1% [0.7–3.1], median [interquartile range]) in comparison with healthy individuals (3.5% [2.1–4.8]). Regarding CD3, we measured a highly significant decrease of its expression on both αβ and γδ T lymphocytes from patients (p < .005), whereas the CD3 mean fluorescence intensities ratio (γδ/αβ) was not affected: 2.2 [2.1–2.4] and 2.1 [1.9–2.3] in healthy individuals and septic patients, respectively. The magnitude in the decrease of CD3 expression was thus similar in αβ and γδ cells, suggesting a common down-regulation mechanism for both T-cell lineages.Combined with a reduced percentage of monocytes expressing human leukocyte antigen-DR, a reduced CD3 expression may be involved in the failure of antigen presentation depicted after septic shock, whereas the diminished percentage of circulating γδ T cells could be partly responsible for the elevated incidence of secondary infections. These two observations constitute additional pieces of the complex puzzle of sepsis-induced immunosuppression.