Nuclear factor-κB activation in peripheral blood mononuclear cells in children with sepsis


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Abstract

Objective:To determine the activation of nuclear factor-κB in peripheral blood CD14+ monocyte/macrophages and CD3+, CD4+, and CD8+ T cells in children with sepsis.Design:Observational study.Setting:University hospital.Patients:Twenty-six children with sepsis (nine females and 17 males, aged between 10 days and 15 yrs; median, 4.3 yrs) on admission to our hospital between August 1999 and November 2005.Interventions:None.Measurements and Main Results:The percentages of peripheral blood CD14+ monocyte/macrophages and CD3+, CD4+, and CD8+ T cells exhibiting nuclear factor-κB activity were determined by flow cytometry. In addition, relationships among the degree to which nuclear factor-κB was activated, serum levels of cytokines (interferon-γ, tumor necrosis factor-α, interleukin-2, interleukin-4, interleukin-6, and interleukin-10), and clinical variables were analyzed. The percentage of cells exhibiting nuclear factor-κB activity was increased among CD14+, CD3+, CD4+, and CD8+ cells in the sepsis group and was significantly higher among CD14+, CD3+, and CD4+ cells of the patients with severe sepsis (n = 9) than those of patients with nonsevere sepsis (n = 17). The percentage of cells exhibiting nuclear factor-κB activity was significantly higher among CD14+ cells than CD3+ cells in the patients with severe sepsis. In addition, this percentage was significantly higher among CD14+ cells than CD3+, CD4+, and CD8+ cells in septic patients who had positive blood cultures (n = 16). Serum interleukin-6 levels were correlated with the percentages of CD14+, CD3+, CD4+, and CD8+ cells exhibiting nuclear factor-κB activity, and serum IL-10 levels were correlated with the percentages of CD14+, CD3+, and CD4+ cells exhibiting nuclear factor-κB activity.Conclusions:Nuclear factor-κB in peripheral blood mononuclear cells was activated in children with sepsis and was related the severity of sepsis.

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