The Effect of Octreotide on Healing of Injured Colonic Anastomosis with Immediate Postoperative Intraperitoneal Administration of 5-Fluorouracil

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Purpose:This study was designed to investigate the effect of octreotide on side effects of immediate usage of 5-fluorouracil after colonic anastomosis.Methods:Forty male Wistar rats were randomly assigned into four groups and underwent standardized left colonic anastomosis. The rats served as control or received intraperitoneal 5-fluorouracil (20 mg/kg daily), subcutaneous octreotide (20 μg/kg daily), or both. Diarrhea and wound complications were noted during the experiment. The colonic anastomoses were assessed for healing on postoperative Day 7 by determining the anastomotic bursting pressure, performing histologic examination, and measuring the tissue hydroxyproline content, serum malondialdehyde, and nitric oxide levels. Intraperitoneal adhesions and anastomotic leakage were also noted.Results:No statistical significant difference was found between the control and octreotide groups for each of the parameters measured. Immediate 5-fluorouracil use resulted with higher adhesion score (P= 0.002), significant depression in anastomotic bursting pressure (P= 0.0001), histopathologic score (P= 0.0001), hydroxyproline content (P= 0.0001), and increasing nitric oxide (P= 0.0001) and malondialdehyde levels (P= 0.0001) compared with the control group. Diarrhea was seen in 80 percent of the 5-fluorouracil group but in neither the control nor octreotide groups (P= 0.0001 for each comparison). However, all these parameters were ameliorated by use of concomitant octreotide and 5-fluorouracil (P= 0.019,P= 0.023,P= 0.0001,P= 0.006,P= 0.0001, andP= 0.013, respectively). In addition, diarrhea was found to be prevented (P= 0.0001).Conclusions:The results of this study showed that concomitant octreotide use might prevent the side effects of 5-fluorouracil, such as diarrhea, postoperative adhesion, and delaying the anastomotic healing parameters. In addition, it might reduce tissue damage and inflammation.

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