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Materials and Methods: Anesthesia was induced and maintained with thiopentone sodium. Mean arterial pressure (MAP) and aortic blood velocity (ABV) measured by a Doppler flow probe positioned around the aorta were continuously monitored. A Clark-type electrode and 5 microdialysis probes were implanted in both quadriceps to monitor tissue O2 partial pressure (PtiO2) and lactate (L) and pyruvate (P) interstitial concentrations. Changes in tissue blood flow were estimated by the ethanol clearance technique1 assessed by the ratio of ethanol concentration in the dialysate divided by a known concentration of ethanol added to the microdialysis perfusate (Cout/Cin). After stabilization for 120 minutes, the animals were randomized to the AS group (n = 12) or to the NCP group (n = 12). OVA or NCP was injected at Time 0. Values are expressed as mean ± SEM. Intra and between groups comparison were achieved using ANOVA followed by Fisher's test when appropriate (p < 0.05 was considered as significant).
Results and Discussion:
Despite a similar MAP reduction in both groups without significant modification in ABV, AS was characterized by a rapid and dramatic decrease in PtiO2 resulting from a 50% decrease in muscle blood flow1. This decrease was associated with an anaerobic metabolism attested to by an increase in interstitial lactate, a decrease in interstitial pyruvate and therefore an increase in L/P ratio.
Conclusion: AS was characterized by muscular hypoxia and anaerobic metabolism. This could be explained by redistribution of blood flow to other organs in AS.
Acknowledgement: Supported by an institutional SFAR grant.
