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Excerpt
Materials and Methods: After approval, in Trial 1 30 FR and 30 WN rats of the same age received antibiotic prophylaxis and underwent peritoneal infection on day 20. In Trial 2 20 FR rats were weight matched to 20 WN, but younger rats. All had i.v. antibiotic prophylaxis with cefuroxime 3mg/metronidazole 10 mg/kg 1 h before surgery and peritoneal stool infection. In 10 rats of Trial 2, plasma cytokines TNF-α, IL-6 and MIP-2 were measured (ELISA) 1 h after infection. Primary endpoint was the 120 hour survival rate analysed using Chi-square test. Cyto-kine data were analysed with the Kruskal-Wallis test and post hoc Bonferroni correction. Means + SEM are shown.
Results and Discussions
FR rats were more resistant to infection than WN rats of same age (others found that FR mice had less neoplasms, auto-immune disease and lived longer compared with WN controls, 1). However this advantage disappeared when we compared weight-matched rats in Trial 2, but pro-inflammatory cytokines were reduced in FR rats (as in 2).
Conclusion(s): Food restriction per se appears to be no independent risk factor for postoperative sepsis. Immuno-modulatory effects should be further evaluated.
