Imaging plates sensitive to beta rays were used to obtain the images of 90Sr in tooth samples taken from mammals collected in contaminated areas of the former Soviet Union. The average concentrations of 90Sr in the samples were determined by comparing the intensities of the luminescence using a single crystal of KCl. The results showed that the determined 90Sr concentration has a positive correlation with the soil contamination levels in the South Ural region. Tooth samples from both inside of the Semipalatinsk nuclear test site and the villages nearby have detectable amounts of 90Sr, indicating the possible presence of residual soil contamination. The present study demonstrates that using imaging plates is a very sensitive method to detect 90Sr in teeth as well as to estimate low-level 90Sr contamination in soil.