Burn Injuries in Rats Upregulate the Gene Expression of the Ubiquitin-Conjugating Enzyme E214k in Skeletal Muscle


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Abstract

Burn injuries are associated with muscle cachexia, which mainly reflects protein breakdown in the ubiquitin-proteasome pathway. Ubiquitination of proteins degraded by this mechanism is regulated by multiple enzymes, including the 14-kd ubiquitin-conjugating enzyme, E214k. In this study, burn injuries in rats resulted in increased levels of the 1.2 kilobase E214k transcript in the white, fast-twitch extensor digitorum longus muscle with no changes or only minor changes in the red, slow-twitch soleus muscle, liver, and kidney. The results provide the first evidence that burn injuries upregulate the gene expression of E214k in skeletal muscle and suggest that ubiquitin-proteasome-dependent muscle protein breakdown after thermal injuries may, at least in part, be regulated by E214k. (J Burn Care Rehabil 2000;21:528-34)

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