Effect of nuclear factor kappa B inhibition on L-NAME-induced hypertension and cardiovascular remodelling

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Abstract

Objective

We aimed to analyze effects of nuclear factor-kappa B (NF-κB) inhibition on blood pressure (BP) regulation and cardiovascular remodelling.

Design

Adult 12-week-old male Wistar Kyoto rats (WKY) were treated with the nitric oxide synthase (NOS) inhibitor NG-nitro-L-arginine methyl ester (L-NAME, 40 mg/kg/day) for seven weeks. From the fourth week of L-NAME treatment, the NF-κB inhibitor lactacystin (1 μg/kg) was applied once a week. Furthermore, age-matched WKY received L-NAME or lactacystin alone for 7 or 3 weeks, respectively.

Methods

Total NOS activity was determined in the left ventricle (LV) and aorta. The concentration of conjugated dienes, fibrosis, and collagen I and III levels were determined in the LV. The cross-sectional area (CSA) and wall thickness to internal diameter ratio (WT/ID) were measured in the aorta.

Results

L-NAME treatment increased BP significantly (145 ± 2 mmHg vs. 110 ± 1 mmHg in controls). The addition of lactacystin resulted in further significant increase in BP (161 ± 3 mmHg). Similarly, lactacystin potentiated the increased conjugated dienes concentration induced by L-NAME. Whereas L-NAME alone did not affect NOS activity, the addition of lactacystin decreased it in both tissues investigated. The addition of lactacystin did not affect LV hypertrophy, fibrosis, and collagen I and III, already increased by L-NAME; however, it further amplified CSA in the aorta increased by L-NAME alone. WT/ID increased significantly only after the addition of lactacystin.

Conclusion

Decreased NOS activity along with increased oxidative load may be responsible for decreased NO bio-availability and further BP increase after NF-κB inhibition in L-NAME-induced hypertension. Increased CSA and WT/ID could contribute to this hypertensive process.

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