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A continuous 24-h esophageal pH monitoring was performed in 283 asymptomatic infants between 5 days and 15 months old. Several parameters (reflux index, duration of the longest reflux episode, number of reflux episodes in 24 h, number of reflux episodes >5 min in 24 h) were studied in different groups of infants according to their age: 5–15 days old, 24–37 days old, 3.5–4.5 months old, 5.5–6.5 months old, 7.5–8.5 months old, 14–16 months old. For all parameters we obtained statistically significant different results in infants younger and older than 4 months. The 24-h esophageal pH monitoring is an investigation technique in physiological circumstances in order to establish normal ranges for gastroesophageal reflux in asymptomatic infants.