Issn Print: 0195-9131
Publication Date: 1996/05/01
EFFECT OF HIGH ALTITUDE AND SEA LEVEL TRAINING ON PROTEIN OXIDATION IN SKELETAL MUSCLE OF RATS 289
Z. Radák; K. Asano; H. Ohno; K. Lee; S. Nakamura; H. Nakamoto; S. Goto
Excerpt
High altitude exposure and physical training causes inverse effects on free radical formation and on the activities of antioxidant enzymes. Therefore, the purpose of the present study was to investigate the combined effects of high altitude exposure and physical training on skeletal muscle antioxidant enzyme status, lipid peroxidation and protein oxidation. Thirty male Wistar rats were assigned to three groups such as, control (C), normoxic trained (NT), and simulated high altitude (4000m) trained (HT). The relative training intensity of NT (1 h running 15 m/min at grade of 10%, 5/wk, 4wk) and HT (1 h, 15 m/min, 5/wk, 4wk) rats were the same, respectively. White (WQ) and red (RQ) parts of quadriceps muscle were measured. The activity of Cu, Zn-superoxide dismutase(SOD) was not changed in either groups. The activity of Mn-SOD showed significant increase in WQ muscle in NT and HT groups, moreover it is also increased in the RQ muscle of HT rats. The activity of catalase remained unchanged and the activity of glutathione peroxidase tend to decreases in the muscle of HT rats. Malondialdehyde and lipid hydroperoxides levels were unaltered. Carbonyl content, measured by immunodetection of protein-bound 2,4-Dinitrophenylhydrazones (DNP) using Western Blot analysis, showed an increase in RQ muscle of HT rats. The data obtained indicate that, physical training at high altitude induce an increase in activity of Mn-SOD, and this increase is not followed by the increases in activity of peroxidease enzymes. It is possible that the increased number of H2O2 molecule reacted with iron causing protein oxidation. The mechanism of lipid and protein oxidation seems to be different in vivo conditions. Moreover, protein oxidation also could be a part of the controlling mechanism of the cell protein turnover and it might relate to the usage of proteins.
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