Cytokine and Oxidative Responses to Maximal Cycling Exercise in Sedentary Subjects


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Abstract

Introduction:The simultaneous determination of the time course and magnitude of oxidative stress indicators and cytokine changes elicited by maximal incremental exercise has not yet been published for healthy sedentary subjects.Purpose:The determination of normal exercise-induced changes in oxidant-antioxidant status and plasma cytokine represents a fundamental step before exploring patients suspected of altered biochemical responses.Methods:Fifteen healthy sedentary subjects performed an incremental cycle exercise until volitional exhaustion with measurement of maximal oxygen uptake (V˙O2max), two cytokines (IL-6 and TNF-α), and three indicators of oxidative stress (plasma thiobarbituric acid reactive substances (TBARS), reduced erythrocyte glutathione (GSH), and reduced plasma ascorbic acid (RAA)).Results:At V˙O2max, we noted a significant increase in plasma IL-6 and TNF-α concentrations, concomitant with the decrease in plasma RAA level. Besides, the plasma TBARS increase and erythrocyte GSH decrease respectively occurred at the 5th and 10th minutes of recovery. The exercise-induced variations of all blood indicators were completed within the 20th minute of the recovery period. We found significant positive correlations between V˙O2max and the peak increases in IL-6 (but not TNF-α) and TBARS. The corresponding variations of IL-6 and TBARS were also correlated.Conclusion:This study indicates that blood samples for analyses of changes in both oxidant-antioxidant status and cytokine levels in response to maximal cycling exercise must be performed within the first 20 min of the postexercise recovery period.

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