DOI: 10.1097/PRS.0000000000000927

,  

,  

PMID: 25626837

Issn Print: 0032-1052

Publication Date: 2015/02/01


Print

A Novel and Effective Strategy for the Isolation of Adipose-Derived Stem Cells: Minimally Manipulated Adipose-Derived Stem Cells for More Rapid and Safe Stem Cell Therapy

Nicolas Bertheuil; Benoit Chaput

    loading  Checking for direct PDF access through Ovid

Excerpt

We read with great interest the article entitled “A Novel and Effective Strategy for the Isolation of Adipose-Derived Stem Cells: Minimally Manipulated Adipose-Derived Stem Cells for More Rapid and Safe Stem Cell Therapy” by Raposio et al.1 Foremost, we want to congratulate the authors for this work and this interesting concept. Indeed, these authors demonstrate the possibility of adipose-derived stromal vascular fraction graft without digestion of the tissue by proteolytic enzymes and, more importantly, without expensive specific devices.2 This is the second study that reports this possibility after the recent work conducted by Tonnard et al.3 However, in our opinion, both studies are insufficient for demonstrating that the transplanted cells are mesenchymal stromal cells.
Indeed, the authors did not demonstrate the stemness of the injected stromal vascular fraction cells. Mesenchymal stromal cells have been known for four decades and were initially isolated from the bone marrow. Within the adipose tissue, mesenchymal stromal cells called adipose-derived stromal cells were first reported in 2001 by Zuk et al.4 These cells were defined in vitro by several criteria5:
In our opinion, it is necessary to demonstrate that the cells produced by this method meet the in vitro criteria of the mesenchymal stromal cells. Moreover, it would be interesting to compare the adipose-derived stromal cell yield obtained with these mechanical methods to the cells obtained with specific devices. It is important, because if the cell yield is equivalent, this will reduce the cost of the stromal vascular fraction–grafted cells, which could be extremely interesting in regenerative medicine.
In conclusion, the authors have limited their investigations to phenotypic characterization without realizing differentiation in the mesodermal lineage and colony-forming unit-fibroblast experiment. It seems insufficient to demonstrate that the cells obtained are adipose-derived stromal cells.
    loading  Loading Related Articles