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Adipose extracellular matrix/stromal vascular fraction gel: a novel adipose tissue-derived injectable for stem cell therapy

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Abstract

Background

Adipose-derived stem cells (ASCs) and other stromal vascular fraction (SVF) cells were used more often for stem cell therapy, even though limitations such as poor cell retention rate, complicated and expensive isolation processes, and the use of specific laboratory equipment need to be overcome.

Methods

Here, we developed a novel but simple method for generating an injectable mixture of SVF cells and native adipose extracellular matrix (ECM). It is a purely mechanical process in which lipoaspirate is processed into an “ECM/SVF-gel”. The standard processing procedure was established using quantized tests. The therapeutic potential of ECM/SVF-gel for wound healing was then tested in an animal model.

Results

ECM/SVF-gel derived from lipoaspirate and processed using a standard Coleman technique, followed by 1 min of mechanical processing by passage back and forth between two 10-ml syringes at a flow rate of 10 ml/sec, showed the highest ASC and endothelial cell density (1.9 ± 0.2 × 105 cells/ml and 7.7 ± 2.4 × 104, respectively). The SVF cells within the ECM/SVF-gel also showed potential for multipotent differentiation similar to that of cells from normal fat samples. Additionally, the ECM/SVF-gel showed better therapeutic results than SVF cell suspension when used to treat a nude mouse model of wound healing.

Conclusions

ECM/SVF-gel is an autologous injectable derived of native ECM and is a functional cellular component generated using a simple mechanical process. As such, it may offer a novel mode of tissue repair suitable for clinical application in stem cell therapies.

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