Issn Print: 0275-004X

Publication Date: 2003/06/01


Print

Letter to the Editor

Matthew Russell; Philip J. Polkinghorne

    loading  Checking for direct PDF access through Ovid

Excerpt

Jabbour and Jabbour1 are to be 1congratulated for highlighting a method for collection of pure vitreous samples during vitreous biopsy. Their technique enables controlled aspiration of the sample, which easily surpasses the conventional method of aspiration into a syringe.2
Our group has similarly developed a technique for collecting pure vitreous samples, which also utilizes the controlled aspiration pressure induced by the vitrector. We believe that it is important to maintain the intraocular pressure and perform a simultaneous air exchange during the biopsy. The vitreous sample is collected in a gassterilized test tube by means of two 20-gauge Venflon intravenous cannulae. A male connector and a malefemale connector placed in the aspiration tubing at a convenient point complete the circuit.
The maintenance of intraocular pressure during sampling not only provides a degree of protection against the complications of hypotony but also facilitates the collection of larger volumes of vitreous. Furthermore, our technique reduces the risk of contamination and enables the laboratory staff to divide and prepare the sample under controlled laboratory conditions.
    loading  Loading Related Articles