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To study the effect of retained subretinal perfluorocarbon liquid (PFCL) on retinal function.Scanning laser ophthalmoscope microperimetry was performed on four eyes with retained subretinal PFCL after vitreoretinal surgery for complicated retinal detachments with and without proliferative vitreoretinopathy.Scotomas to the highest intensity stimulus were noted in the area of the subretinal PFCL in all four eyes. All scotomas encompassed approximately the same area as the retained PFCL droplets. In the area vacated by a migrating PFCL droplet in one eye, the highest intensity stimulus could be perceived, but a relative scotoma (to a lower intensity stimulus) was present.There is a local reduction in retinal function in the area of retained subretinal PFCL. There may be partial recovery of retinal function in an area vacated by the subretinal PFCL. Subretinal PFCL beneath the fovea or at risk for migration beneath the fovea should be considered for removal.