Therapeutic Drug Monitoring. 17(6):700–703, DECEMBER 1995
PMID: 8588244
Issn Print: 0163-4356
Publication Date: December 1995
Elucidating the Mechanism of Action of the Immunosuppressant 15-Deoxyspergualin
Steven Nadler;Ashley Eversole;Mark Tepper;Jefferey Cleaveland;
+ Author Information
Bristol-Myers Squibb Pharmaceutical Research Institute, Seattle, Washington, U.S.A.
Abstract
15-Deoxyspergualin (DSG) is an immunosuppressive agent currently in Phase I/II clinical trials. We have previously shown that DSG specifically interacts with Hsc70, a member of the heat shock protein 70 family. In this article we show that DSG appears to bind either kinetically or to a different site on Hsc70 from that of peptides. In addition, we show that DSG inhibits the localization of Hsp70 into the nucleus in response to heat shock. Finally, data are presented showing that there is a correlation between decreases in the transcription factor nuclear factor kappa B and K light chain expression in response to varying doses of DSG.
