AN EXTENSION OF THE51Cr-RELEASE ASSAY FOR THE ESTIMATION OF MOUSE CYTOTOXINS


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Abstract

SUMMARYThe 51Cr-release assay for measurement of cytotoxic reactions, with its many advantages, has hitherto been applicable only to mouse cell types which occur free from contamination with erythrocytes. A simple method is presented which can selectively lyse contaminating erythrocytes from tissue cell preparations without damaging the tissue cells. The tissue cells can then be labelled and tested by the 51Cr-release cytotoxicity assay. The usefulness of the method is shown by demonstrating its success with suspensions of mouse spleen cells and with a hemorrhagic ascites sarcoma.

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