Cryopreserved Agarose-Encapsulated Islets As Bioartificial Pancreas: A Feasibility Study

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Abstract

Background.

A bioartificial pancreas in which islets of Langerhans (islets) are encapsulated within a semipermeable membrane, such as agarose, has been proposed for treating type I diabetic mellitus. However, the long-term storage for providing a convenient and easily accessible supply still remains an issue. We investigated cryopreservation as a potential method of long-term storage for agarose-encapsulated islets (Mic-islets).

Methods.

The morphology, insulin secretion, and histochemical staining of cryopreserved Mic-islets were analyzed. Streptozotocin-induced diabetic mice were transplanted intraperitoneally with 1000, 2000, and 3000 Mic-islets after cryopreservation in KYO-1 vitrification solution. Blood glucose levels were measured and immunohistochemical analyses were performed at 41 days posttransplantation.

Results.

Transplanted cryopreserved Mic-islets restored normoglycemia in diabetic mice. The mean (±SD) normoglycemic periods were 32.0±13.2 days and 46.3±13.3 days for recipients of 1000 (n=5) and 2000 (n=4) cryopreserved Mic-islets, respectively, whereas the mean normoglycemic period was 53.2±16.7 days for recipients of 1000 noncryopreserved Mic-islets (n=7). These data indicate that cryopreserved Mic-islets transplanted as a bioartificial pancreas successfully controlled blood glucose levels for extended periods.

Conclusion.

Cryopreserved agarose-encapsulated islets could successfully control the blood glucose level for a long period as a bioartificial pancreas.

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