ENDOGENOUS OPIATES DO NOT MODULATE LPS-INDUCED ALTERATIONS IN CARBOHYDRATE METABOLISM

Abstract

This study aimed to determine the contribution of endogenous opiates to the hormonal and glucose metabolic response to lipopolysaccharide (LPS). Rats were infused with naloxone (NAL) (32 μg/h) for 2 h prior to the injection of LPS (100 μ/100 g body weight) and hemodynamic, hormonal, and metabolic parameters were determined. NAL produced no detectable alterations in any of the parameters assessed. LPS transiently decreased (26%) mean arterial blood pressure (MABP), and increased plasma glucose concentration (100-130%), glucose rate of appearance (50-100%), and glucose rate of disappearance (50-100%). NAL did not alter the LPS-induced drop in mean arterial blood pressure or the glucose response to LPS. LPS reduced plasma insulin (54%), and increased glucagon (270%), corticosterone (180%), and tumor necrosis factor concentrations in plasma (peak 3200-4600 pg/mL at 90 min), with no modification by NAL pretreatment. These results suggest a lack of involvement of endogenous opiate pathways in the glucose metabolic and hormonal responses to LPS.