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The purposes of this study were to determine test-retest reliability of the one-leg hop test when using hop distances (cm) and the hop index (distance hopped on the involved leg divided by the distance hopped on the noninvolved leg x 100) as criterion measurements and to compare two-data analysis strategies (the mean of three hops vs. the longest hop). Twenty-two men and 16 women who had undergone anterior cruciate ligament (ACL) reconstruction (mean time between surgery and testing 22 ± 7 years) performed the one-leg hop test on two occasions within a 2 week period. Test-retest reliability, determined using intraclass correlation coefficients (ICCs) (Type 2,1), was acceptable (ICCs > 0.75). Although hop distances calculated using the longest hop were significantly greater than those determined using the mean of three hops (p < 0.01), hop indexes were not affected by data analysis strategy. The clinical significance of these findings is that when the one-leg hop test is used to assess patients with ACL reconstruction, reliable hop distances and hop index scores can be produced on one test occasion.