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Due to the emphasis on preventive sports medicine, it has become increasingly important to identify physiological indices of athletic performance and predisposition to injury. Although physiological testing is typically used to assess physical status of athletes in numerous sports, no research has been published that addresses the physiological profile of college lacrosse athletes. Thus this study sought to quantify body composition, maximal aerobic power, anaerobic capacity, blood chemistry, and coronary risk factors on 30 college male club-lacrosse athletes. Data were grouped by player position (attack, n = 5; defense, n = 8; goalie, n = 2; midfield, n = 15) and skill level (1st team, n = 14; 2nd team, n = 16). Results indicated the lacrosse players were above average in some indices of maximal aerobic power during exercise stress testing. They had higher maximal power, mean power, and total work output, collectively, than reported for other college athletes. Analysis of body composition revealed a mean body fat of 15% for the entire sample. Mean resting blood chemistries were within normal limits. Based on their exercise response, body composition, and blood lipid profile, these athletes are at low risk for developing coronary heart disease. They also exhibit high anaerobic capacity reflective of this sport.