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Garnacho-Castaño, MV, Domínguez, R, Ruiz-Solano, P, and Maté-Muñoz, JL. Acute physiological and mechanical responses during resistance exercise at the lactate threshold intensity. J Strength Cond Res 29(10): 2867–2873, 2015—The purpose of this study was to examine acute metabolic, mechanical, and cardiac responses to half-squat (HS) resistance exercise performed at a workload corresponding to the lactate threshold (LT). Thirteen healthy subjects completed 3 HS exercise tests separated by 48-hour rest periods: a maximal strength or 1 repetition maximum (1RM) test, an incremental load test to establish the % 1RM at which the LT was reached, and a constant load test at the LT intensity. During the last test, metabolic, mechanical, and cardiac responses were monitored respectively through blood lactate concentrations, height (H), average power (AP) and peak power (PP) recorded in a countermovement jump test, and heart rate (HR). During the constant load test, lactate concentrations and HR remained stable whereas significant reductions were detected in H, AP, and PP (p ≤ 0.05). Only low correlation was observed between lactate concentrations and the H (r = 0.028), AP (r = 0.072), and PP (r = 0.359) losses produced. Half-squat exercise at the LT elicits stable HR and blood lactate responses within a predominantly aerobic metabolism, although this exercise modality induces significant mechanical fatigue.