DOI: 10.1097/01.WON.0000319421.27848.44
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Issn Print: 1071-5754
Publication Date: 2008/05/01
THE ACTIVITY OF A SILVER HYDROCELLULAR FOAM DRESSINGS AGAINST A BROAD SPECTRUM OF WOUND PATHOGENS USING A DYNAMIC SHAKE FLASK METHOD: 2404
Excerpt
In this study, log reduction using a dynamic 5 ml shake flask test was used to assess the activity of silver hydrocellular Adhesive and Nonadhesive dressings against Pseudomonas aeruginosa, Methicillin resistant Staphylococcus aureus (MRSA), Escherichia coli, Vancomycin Resistant Enterococci (VRE), Candida albicans, Bacteroides fragilis, Clostridium perfringens and Prevotella melaninogenica. This is an in vitro method where a 5 ml suspension of organism is used to challenge a dressing containing an active over a specified time period. Six replicate pieces of 2 cm ÷ 2 cm dressing are inoculated with 5 ÷ 105 CFU/ml of a relevant organism, to reflect a clinical situation. Samples are incubated under dynamic conditions at 32°C for 0h and 24 h. The dynamic nature of the test ensures good contact with the bacteria and the dressing, whilst being emulative of movements in dressing wear. Following incubation, the dressings are removed and the resulting suspension neutralized to arrest the silver activity. Subsequently numbers of surviving bacteria remaining are assessed using standard microbiology techniques. The Log reduction for the test dressings is calculated by subtracting the mean value obtained for the control. In activity testing, an active such as silver may be considered cidal if it achieves log10 reductions of 3 log10 or greater. Both the Adhesive and Nonadhesive formats of the silver hydrocellular foam dressing achieved mean log10 reductions greater than 3 log10 CFU/sample within 24 hours against a broad spectrum of wound pathogens, including Gram positive and Gram negative bacteria, antibiotic resistant bacteria, anaerobes and yeast.
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