Homing ability of bone marrow mesenchymal stem cell transplantation in acute hepatic injury rats*,⋆

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Abstract

BACKGROUND:

There is no unified consensus in studies concerning differentiation of bone marrow mesenchymal stem cells (BMSCs) following transplantation and what is the degree of BMSCs transplantation in repair of hepatocytes.

OBJECTIVE:

To observe homing and differentiation of BMSCs via caudal vein transplantation in acute hepatic injury rats.

DESIGN, TIME AND SETTING:

The cytological in vivo study was performed at the Central Laboratory of Lanzhou University from December 2007 to June 2008.

MATERIALS:

A total of 47 clean male Sprague Dawley rats aged 6-8 weeks were used. Five rats were utilized for BMSCs preparation. The remaining 42 rats were randomly assigned into normal liver BMSCs group (n=15), injured liver BMSCs group (n=14), and injured liver saline group (n=13).

METHODS:

Rat models of acute hepatic injury were established by intraperitoneal injection of D-aminogalactose in the injured liver BMSCs group and injured liver saline group. 24 hours following model induction, 1 mL the third passage of BMSCs suspension containing (1.5-2.0)×106 cells labeled with BrdU was injected into rats in the injured liver BMSCs group and normal liver BMSCs group via the caudal vein. Rats in the injured liver saline group were infused with an equal volume of saline.

MAIN OUTCOME MEASURES:

Hepatic functional recovery following transplantation, and results of immunohistochemistry in the liver were measured.

RESULTS:

Compared with that at 24 hours following model induction, there were no significant changes in serum glutamate pyruvate transaminase activity in the normal liver BMSCs group and injured liver saline group at 2 and 3 weeks following transplantation (P > 0.05). Serum glutamate pyruvate transaminase activity significantly decreased in the injured liver BMSCs group (P < 0.05), and hepatic function recovered well. Compared with the normal liver BMSCs group, number of Brdu+ cells significantly increased in the injured liver BMSCs group (P < 0.01), and mostly distributed in the portal region and surrounding the central veins. With time prolonged, the number of Brdu+ cells gradually reduced.

CONCLUSION:

BMSCs transplanted through tail vein can promote the recovery of hepatic function in the acute hepatic injury rats, and can differentiate in the injured and normal livers. The homing and differentiation may be associated with the degree of liver injury.

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