Effects of muscle-derived stem cell transplantation on denervated gastrocnemius atrophy in rats*,⋆

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Abstract

BACKGROUND:

Studies concerning muscle-derived stem cell transplantation mainly focused on treatment of muscular dystrophy. There are no studies on muscle-derived stem cells for treatment of denervated amyotrophy combined therapy.

OBJECTIVE:

To explore effects of muscle-derived stem cell transplantation on denervated gastrocnemius atrophy.

DESIGN, TIME AND SETTING:

The randomized controlled animal study was performed at the Shenyang Medical College from February 2007 to July 2008.

MATERIALS:

A total of 19 adult healthy male Wistar rats were supplied by Animal Experimental Center, Shenyang Medical College.

METHODS:

Triceps brachii was sterilely collected from 3 rats, and muscle-derived stem cells were isolated in vitro, and purified by adherence method. The tibial nerve of right posterior limb was cut in remaining 16 rats to establish animal models of denervated gastrocnemius. In the cell transplantation group, 0.2 mL DAPI-labeled muscle-derived stem cell suspension was infused into the denervated gastrocnemius. The same volume of saline was injected into the model control group, for 4 weeks.

MAIN OUTCOME MEASURES:

Computer biological signal collection processing system was used to measure recovery rate of threshold intensity of gastrocnemius and the optimal intensity recovery rate. Electronic balance was utilized to calculate residual rate of wet weight. Image analysis system was used to measure residual rate of cross section area.

RESULTS:

Compared with the model control group, the recovery rate of threshold intensity of denervated gastrocnemius and the optimal intensity recovery rate were significantly lower in the cell transplantation group (P < 0.01, P < 0.05). Residual rate of wet weight and residual rate of cross section area were significantly higher (P < 0.01).

CONCLUSION:

Muscle-derived stem cell transplantation can delay atrophy process of denervated skeletal muscle in rats.

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