Osteoblastic growth peptide regulates proliferation of human periodontal ligament cells and alkaline phosphatase activities*,☆

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Abstract

BACKGROUND:

Osteogenic growth peptide (OGP) is a newly discovered growth factor to promote mitosis and bone formation, it is of great significance on the repair process of tissue injury.

OBJECTIVE:

To elucidate the effect of OGP on proliferation of human periodontal ligament cells cultured in vitro and alkaline phosphatase activities.

DESIGN, TIME AND SETTING:

A randomized control observational experiment based on cells was completed at the Key Laboratory for Oral Biomedical Engineering by the State Ministry of Education from February 2006 to June 2006.

MATERIALS:

Periodontal ligament tissues were harvested from periodontal health caries-free fresh first molar, requiring extraction because of clinical orthodontic needs. OGP was purchased from Sigma Company.

METHODS:

Human periodontal ligament cells cultured in vitro were added into medium containing various concentrations of OGP (10−11, 10−10, 10−9, 10−8 and 10−7 mol/L); Cell proliferation was determined using MTT method; Cell cycle was assayed with flow cytometry; Alkaline phosphatase activity was examined with enzyme-linked immunosorbent assay.

MAIN OUTCOME MEASURES:

Cellular proliferation at the 1st, 2nd, 3rd days of cell culture; Alkaline phosphatase activity at the 6th day of cell culture.

RESULTS:

All OGP concentrations used (10−11-10−7 mol/L) were effective to promote the proliferation of human periodontal ligament cells (P < 0.05), and the maximum response was obtained with 10−9 mol/L, at which of OGP could notably increase the percentage of cells at S phase and thus accelerating cellular proliferation. OGP at various concentrations (10−11-10−7 mol/L) could significantly enhance alkaline phosphatase activity (P < 0.05) and the optimal concentration was 10−9 mol/L.

CONCLUSION:

OGP stimulates proliferation of human periodontal ligament cells and alkaline phosphatase activity.

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