Interventional effect of diacerein on bone mass loss in ovariectomized rats

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Abstract

BACKGROUND:

Diacerein is a new kind of interleukin-1 block agent. Researches regarding intervention effect of interleukin correlation factor on increase of bone absorption in postmenopausal osteoporosis are few.

OBJECTIVE:

To investigate the intervention effect of diacerein on bone mass loss in ovariectomized (OVX) rats.

DESIGN, TIME AND SETTING:

The complete randomized controlled experiment was performed in the Laboratory of Experimental Animal Center of Chongqing Medical University from August to November 2008.

MATERIALS:

Forty female, 3-month-old, Sprague Dawley rats were randomly divided into sham operation, OVX, diacerein 10mg, 100 mg/(kg.d) groups, with 10 animals in each group. Anbiding capsule (contains 48.1 mg diacerein in each 50 mg capsule) was produced by Kunming Jida Pharmaceutical Co., Ltd, with batch number of 20070532.

METHODS:

All rats received ovariectomy exception the sham operation group. Rats of sham operation group were only received a little fatty tissue removal around the ovary, and sutured the skin. Diacerein was intragastric administrated at the second day after the ovariectomy in the diacerein 10mg, 100 mg/(kg.d) groups, equivalent volume of normal saline was administrated in the sham operation, OVX groups. Then all rats were killed under anesthesia to collect the blood and skeleton samples after 10 weeks continue medication.

MAIN OUTCOME MEASURES:

Bone mineral density of the right femur and the third lumbar vertebra were determined by Dual-Energy X-ray bone densitometry. The levels of interleukin-1β, interleukin-6 in bone tissue and the level of serum osteocalcin were determined by radio-immunity assay.

RESULTS:

All rats were involved in the final analysis. The levels of bone mineral density in two diacerein groups were higher than that in the OVX group (P < 0.05), which was lower in the OVX group than the sham operation group (P < 0.05). The levels of interleukin-1β, interleukin-6 in bone tissue in the OVX group were higher than that of the sham operation group (P < 0.05), which was lower in two diacerein groups than that in the OVX group (P < 0.05). Compared with the sham operation group, the level of serum osteocalcin in the OVX group was higher (P < 0.05). The differences of serum osteocalcin levels between OVX group and two diacerein groups had not statistically difference (P > 0.05).

CONCLUSION:

The bone loss is obviously 10 weeks later after ovariectomy. Diacerein has intervention effect on bone loss induced by increase of bone absorption in OVX rats, which have no significant effects on bone formation mediated by osteoblast.

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