Association between 29T>C single nucleotide polymorphism of transforming growth factor-beta 1 gene and spinal fusion

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Abstract

BACKGROUND:

Many factors influence spinal fusion, however, there are few reports regarding the association of polymorphism of transforming growth factor-β 1 (TGF-β 1) gene with spinal fusion

OBJECTIVE:

To explore the association of the first exon 29T>C polymorphism of TGF-β 1 gene with spinal fusion.

DESIGN, TIME AND SETTING:

The observation and experiment of gene polymorphism was performed at Molecular Medical Lab, the Affiliated Hospital of Medical College, Qingdao University between July 2007 and May 2008.

PARTICIPANTS:

Two hundred health adults and 210 patients with cervical vertebrae or lumbar bone graft in Shandong peninsula.

METHODS:

The first exon 29T>C polymorphism of TGF-β 1 gene was analyzed by allele-specific PCR and gene sequencing methods in peripheral blood of 210 patients with spinal fusion and 200 healthy controls. The conditions of bone graft fusion were carried out into different grades according to imaging. At the same time, the association of the 29T>C polymorphism of TGF-β 1 with spinal fusion was measured.

MAIN OUTCOME MEASURES:

Whether there is association between the 29T>C Polymorphism of transforming growth factor-β 1 gene and spinal fusion.

RESULTS:

There were the 29T>C polymorphisms of TGF-β 1 gene in two groups, which was no significant difference between the two groups (P=0.707). The distribution of the 29T>C polymorphism of TGF-β 1 gene in the cases was related with the degree of C-spine auto-ilium graft interbody fusion (P=0.001), but was no related with the degree of bone graft fusion inter transverse process of the lumbar vertebra (P=0.647).

CONCLUSION:

TT genotype among 29T>C polymorphisms of TGF-β 1 gene may be a factor which promote C-spine auto-ilium graft fusion. There are many factors which affect bone graft fusions inter transverse process of lumbar vertebra, which needs further explored.

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