Correlation between −866G/A variation in the promoter region of uncoupling protein-2 gene and the risk of type 2 diabetes in population from Beijing*,*,*,⋆

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Abstract

OBJECTIVE:

To explore the relationship between the polymorphism of −866G/A variant in the promoter region of uncoupling protein-2 gene and type 2 diabetic mellitus (T2DM) in the population of Beijing region.

METHODS:

Using polymerase chain reaction- restriction fragment length polymorphism, we tested the distribution of the −866 G/A in promoter region of uncoupling protein-2 gene of 470 sporadic T2DM patients and 217 unrelated healthy Chinese from Beijing. This variant distribution mode was compared with the data in haplotype mapping database. The clinical physical and plasma markers were also compared between different genotypes.

RESULTS:

The frequency of the allele and the genotype had no significant correlation with T2DM (P=0.538, 0.301). The −866 G/A variant distribution mode in this study was close to the mode of Japanese in haplotype mapping database. Stratified analysis and Logistic regression analysis were performed taken gender, waistline and body mass index as covariants. Results showed that the frequency of the allele and the genotype in variant site was still no associated with the development of T2DM. Compared the clinical markers, such as blood pressure, obesity indices (bone mass index, waistline and waist-to-hip ratio), blood glucose indices (glycosylated hemoglobin, fasting blood glucose and blood glucose after meals two hours), lipid and insulin, as well as insulin resistance, there was no significant difference between genotypes.

CONCLUSION:

The distribution of genotype and allele in −866 G/A variant in the promoter region of uncoupling protein-2 gene has no contribution on the T2DM in the population from Beijing region, it is possible not the predisposing genes of the T2DM development in Beijing population.

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